Coding
Part:BBa_K3007025:Design
Designed by: Cheng Li Group: iGEM19_QHFZ-China (2019-10-16)
Expression device of HucR & YgfU: Uric acid detector & uric acid transporter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 32
Illegal EcoRI site found at 1849
Illegal XbaI site found at 135
Illegal PstI site found at 38 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 32
Illegal EcoRI site found at 1849
Illegal PstI site found at 38 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 32
Illegal EcoRI site found at 1849
Illegal BamHI site found at 43
Illegal BamHI site found at 118
Illegal BamHI site found at 982 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 32
Illegal EcoRI site found at 1849
Illegal XbaI site found at 135
Illegal PstI site found at 38 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 32
Illegal EcoRI site found at 1849
Illegal XbaI site found at 135
Illegal PstI site found at 38
Illegal AgeI site found at 1164 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
The key component HucR comes from D. radiodurans, and YgfU comes from E. coli.